| 质粒名称: | pLNCX chick src Y527F |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 13660 |
| 存储实验室: | Joan Brugge |
|
载体骨架基本信息 |
|
| 载体名称: | pLNCX |
| 载体出品公司: | BD Biosciences |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6600 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | src Y527F,src |
| 物种来源: | G. gallus (chicken) |
| 插入大小: | 1900 bp |
| 基因突变: | Y527F. Constitutive active mutant. |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | ClaI（未销毁） |
| 3' 克隆位点: | ClaI（未销毁） |
| 5' 测序引物 | LNCX primer |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pLNCX chick src Y527F was a gift from Joan Brugge (Addgene plasmid # 13660 ; http://n2t.net/addgene:13660 ; RRID:Addgene_13660) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLNCX chick src Y527F 8486 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLNCX chick src Y527F
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8486)
TITLE src plasmids
REFERENCE 2 (bases 1 to 8486)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8486
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 169..548
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 549..752
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 703..723
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 749..773
/label=LNCX
/note="Human CMV promoter, forward primer"
primer_bind complement(3465..3487)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3573..3713
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 3628..3647
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(3728..3745)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3899..4487)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3979..3998)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4658..5518)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5281..5300
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5519..5623)
/gene="bla"
/label=AmpR promoter
primer_bind 5691..5709
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
LTR 6281..6456
/label=5' LTR (truncated)
/note="truncated long terminal repeat from Moloney murine
sarcoma virus"
misc_feature 6519..6718
/label=MMLV Psi
/note="packaging signal of Moloney murine leukemia virus
(MMLV)"
CDS 6919..7335
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 7265..7287
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 7303..7319
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
CDS 7379..8173
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(7433..7452)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 8043..8062
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
ORIGIN
1 gtggggaggc acgatggccg ctttggtcga ggcggatccg gccattagcc atattattca
61 ttggttatat agcataaatc aatattggct attggccatt gcatacgttg tatccatatc
121 ataatatgta catttatatt ggctcatgtc caacattacc gccatgttga cattgattat
181 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
241 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
301 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
361 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
421 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
481 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
541 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
601 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
661 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
721 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga
781 gacgccatcc acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg
841 gccccaagct tatcgataag ctgatcccct gttttgccct tagactattc aagttgcctc
901 tgtggattag ggctggaggc agctcggatg gtctgacggc cagataaggc aggaaagaca
961 gctattggta attgtgaaat acgcttttgt ctgtgtgctg caggagctga gctgactctg
1021 ctggtggcct cgcgtaccac tgtggccagg cggtagctgg gacgtgcagc cgaccaccat
1081 ggggagcagc aagagcaagc ccaaggaccc cagccagcgc cggcgcagcc tggagccacc
1141 cgacagcacc caccacgggg gattcccagc ctcgcagacc cccaacaaga cagcagcccc
1201 cgacacgcac cgcaccccca gccgctcctt tgggaccgtg gccaccgagc ccaagctctt
1261 cgggggcttc aacacttctg acaccgtcac gtcgccgcag cgtgccgggg cactggctgg
1321 cggcgtcacc actttcgtgg ctctctatga ctacgagtcc cggactgaaa cggacttgtc
1381 cttcaagaaa ggagaacgcc tgcagattgt caacaacacg gaaggtgact ggtggctggc
1441 tcattccctc actacaggac agacgggcta catccccagt aactatgtcg cgccctcaga
1501 ctccatccag gctgaagagt ggtactttgg gaagatcact cgtcgggagt ccgagcggct
1561 gctgctcaac cccgaaaacc cccggggaac cttcttggtc cgggagagcg agacgacaaa
1621 aggtgcctat tgcctctccg tttctgactt tgacaacgcc aaggggctca atgtgaagca
1681 ctacaagatc cgcaagctgg acagcggcgg cttctacatc acctcacgca cacagttcag
1741 cagcctgcag cagctggtgg cctactactc caaacatgct gatggcttgt gccaccgcct
1801 gaccaacgtc tgccccacgt ccaagcccca gacccaggga ctcgccaagg acgcgtggga
1861 aatcccccgg gagtcgctgc ggctggaggt gaagctgggg cagggctgct ttggagaggt
1921 ctggatgggg acctggaacg gcaccaccag agtggccata aagactctga agcccggcac
1981 catgtccccg gaggccttcc tgcaggaagc ccaagtgatg aagaagctcc ggcatgagaa
2041 gctggttcag ctgtacgcag tggtgtcgga agagcccatc tacatcgtca ctgagtacat
2101 gagcaagggg agcctcctgg atttcctgaa gggagagatg ggcaagtacc tgcggctgcc
2161 acagctcgtc gatatggctg ctcagattgc atccggcatg gcctatgtgg agaggatgaa
2221 ctacgtgcac cgagacctgc gggcggccaa catcctggtg ggggagaacc tggtgtgcaa
2281 ggtggctgac tttgggctgg cacgcctcat cgaggacaac gagtacacag cacggcaagg
2341 tgccaagttc cccatcaagt ggacagcccc cgaggcagcc ctctatggcc ggttcaccat
2401 caagtcggat gtctggtcct tcggcatcct gctgactgag ctgaccacca agggccgggt
2461 gccataccca gggatggtca acagggaggt gctggaccag gtggagaggg gctaccgcat
2521 gccctgcccg cccgagtgcc ccgagtcgct gcatgacctc atgtgccagt gctggcggaa
2581 ggaccctgag gagcggccca cttttgagta cctgcaggcc ttcctggagg actacttcac
2641 ctcgacagag ccccagttcc agcctggaga gaacctatag atctgcgcga gcaaaattta
2701 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
2761 ttttgcgctg cttcgcatcg ataggcctaa cacgagccat agataaaata aaagatttta
2821 tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctagct
2881 taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga gaagttcaga
2941 tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc
3001 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag
3061 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
3121 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
3181 cgagctcaat aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga
3241 gtcgcccggg tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct
3301 cgctgttcct tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca
3361 tttgggggct cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg
3421 aggtaagctg gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
3481 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
3541 ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
3601 agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
3661 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
3721 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
3781 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
3841 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
3901 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
3961 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
4021 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
4081 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
4141 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
4201 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
4261 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
4321 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
4381 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
4441 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
4501 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
4561 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
4621 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
4681 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
4741 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
4801 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
4861 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
4921 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
4981 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
5041 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
5101 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
5161 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
5221 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
5281 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
5341 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
5401 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
5461 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
5521 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
5581 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
5641 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
5701 tcacgaggcc ctttcgtctt caagaattca taccagatca ccgaaaactg tcctccaaat
5761 gtgtccccct cacactccca aattcgcggg cttctgcctc ttagaccact ctaccctatt
5821 ccccacactc accggagcca aagccgcggc ccttccgttt ctttgctttt gaaagacccc
5881 acccgtaggt ggcaagctag cttaagtaac gccactttgc aaggcatgga aaaatacata
5941 actgagaata gaaaagttca gatcaaggtc aggaacaaag aaacagctga ataccaaaca
6001 ggatatctgt ggtaagcggt tcctgccccg gctcagggcc aagaacagat gagacagctg
6061 agtgatgggc caaacaggat atctgtggta agcagttcct gccccggctc ggggccaaga
6121 acagatggtc cccagatgcg gtccagccct cagcagtttc tagtgaatca tcagatgttt
6181 ccagggtgcc ccaaggacct gaaaatgacc ctgtacctta tttgaactaa ccaatcagtt
6241 cgcttctcgc ttctgttcgc gcgcttccgc tctccgagct caataaaaga gcccacaacc
6301 cctcactcgg cgcgccagtc ttccgataga ctgcgtcgcc cgggtacccg tattcccaat
6361 aaagcctctt gctgtttgca tccgaatcgt ggtctcgctg ttccttggga gggtctcctc
6421 tgagtgattg actacccacg acgggggtct ttcatttggg ggctcgtccg ggatttggag
6481 acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc aacttatctg
6541 tgtctgtccg attgtctagt gtctatgttt gatgttatgc gcctgcgtct gtactagtta
6601 gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc
6661 cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga
6721 agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac
6781 ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg
6841 tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg
6901 tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac
6961 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg
7021 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc
7081 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga
7141 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
7201 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
7261 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
7321 ccttctctag gcgccggaat tccgatctga tcaagagaca ggatgaggat cgtttcgcat
7381 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
7441 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
7501 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
7561 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
7621 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
7681 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
7741 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
7801 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
7861 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
7921 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
7981 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
8041 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
8101 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
8161 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
8221 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
8281 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
8341 caccccgggc tcgatcccct cgcgagttgg ttcagctgct gcctgaggct ggacgacctc
8401 gcggagttct accggcagtg caaatccgtc ggcatccagg aaaccagcag cggctatccg
8461 cgcatccatg cccccgaact gcagga
//
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